Assuming he is eating between 2-3 times a day usually, I would say no. Of course, with only 1 days time we can't really be sure anything is truly wrong. Maybe he is just not feeling well today and is letting his digestion catch up. Without more info and time it would be pretty hard to say whether or not something is wrong.
But, I will still give a theory nonetheless. Assuming you didn't re-pot at least the top half of the soil, there is a chance that he could have ingested something harmful. Has he had a BM since you noticed the lack of eating? Does he appear bloated on his sides/toward his trap? Has any other behavior changed? Drinking habits, sleeping habits, general activity during the day, basking spot, etc...The more info you give, the better we can help.