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Basically you mount your Macro lens like you normally do. Then using the coupler (make sure you buy one that matches the filter threads on both lenses) add the 50mm lens. Keep the aperture on the 50mm lens wide open or else you will get a black ring in your shots (this Aperture does not affect DOF).
You now have a camera with two lenses facing each other.
Adding the 50mm lens, lets you focus closer than your other lens alone will let you.
You operate your Macro lens like you normally do...it is just like adding a close-up filter(or a magnifying glass) on your existing lens.
I think you meant DOF....
DOF is Depth of Field. DOF on any lens is controlled by the Aperture, Focal length and Subject Distance.
The smaller the Apeture (larger number) the more DOF you get.
The closer you are to your subject, the less DOF you get. <<<<This is what you will experience, since you will be much closer to your subject. The ant shot was done with the ant less than 1/2 inch away from the lens.
Dunno if you know Canon gear.. but do ya know if it matters which 50mm you do this with? Kinda neat, might want to try
I use the plastic fantastic for reverse macro coupling.
Check these out for reversed 50 shots:
https://www.chameleonforums.com/veiled-macros-17709/
https://www.chameleonforums.com/panther-macros-18179/
https://www.chameleonforums.com/ho-ho-hornworms-macro-18734/
Good luck!
They can be found at Fleabay.