Parasite ID???

AndrewH

New Member
Any guesses as to what those cells with the 3 nuclei are?

Was thinking coccidia, but was told most cases have 2 or 1 nuclei or a mix. The cells seem to ALL have 3 nuclei.

IMG_5009Mod1.jpg
 
I'm not a vet - but I have seen coccidia over and over (...albeit 10-12 years ago).

that looks like it to me - and there appear to be no more than 2 nuclei per cell - the other thigns seem to me to be organelles, but not nuclei... I think.
 
What magnification was this taken at? (10x, 40x?) Without knowing that I'd say coccidia. They can be found multinucleated with 3-4 nuclei present, somewhat dependent at the angle the oocyst is observed at.
 
Coccidia.....

It looks like coccidia to me. There are more than one species of Coccidia. I'm betting your magnification must be much greater than 40X to see them in this fashion though................Time for Albon.
 
Coccidia?? the mother of alllll parasites...fun times. :eek: Heh. I'll be sure to get some medication for her. Thanks for the expert eyes everyone :)
 
Howdy Andrew,
I took those photos at 400x. The images are also a bit zoomed in when I cropped them so they are even magnified on top of that. I guess I could agree with an ID of coccidia :(. I was hoping that since it didn't look like the perfect coccidia format that maybe you escaped :eek:.

Here's a 400x coccidia photo that I took from another chameleon for comparison:
Coccidia400x.jpg
 
Howdy Andrew,
I took those photos at 400x. The images are also a bit zoomed in when I cropped them so they are even magnified on top of that. I guess I could agree with an ID of coccidia :(. I was hoping that since it didn't look like the perfect coccidia format that maybe you escaped :eek:.

Here's a 400x coccidia photo that I took from another chameleon for comparison:
Coccidia400x.jpg

Thanks Dave :)

yea, looking at the chart you had the other day i didnt see anything else that looked like it. maybe it was just a different strain from the common one normally found. What is the recommended dosage for Albon? and Dave, what was the recommended dosage for Apertex??
 
Coccidia types.............

The original photo that is zoomed and cropped could be Eimeria Coccidia. That type has an oocyst that is subdivided into four sporocysts. The depth of field in the original photo could be "hiding" one or two of the sporocysts in some of the oocysts. Since the oocysts are round and three dimensional you can't have all the sporocysts in focus at the same time. The second photo you have for comparison is classic Isospora Coccidia. That type has an oocyst that is subdivided into two sporocysts. After oocysts of both types have been exposed to air outside the body for a couple of days the sporocysts subdivide and the Coccidia actually becomes infectious. Cryptospordium is another type of Coccidia that is a deadly and dreaded form. You can't see the sporocysts in that type in our microscopes because they are super tiny. I think your cham has Eimeria type Coccidia. But in any case, both Eimeria and Isospora are treated the same way. Hope this helps....
 
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The original photo that is zoomed and cropped could be Eimeria Coccidia. That type has an oocyst that is subdivided into four sporocysts. The depth of field in the original photo could be "hiding" one or two of the sporocysts in some of the oocysts. Since the oocysts are round and three dimensional you can't have all the sporocysts in focus at the same time. The second photo you have for comparison is classic Isospora Coccidia. That type has an oocyst that is subdivided into two sporocysts. After oocysts of both types have been exposed to air outside the body for a couple of days the sporocysts subdivide and the Coccidia actually becomes infectious. Cryptospordium is another type of Coccidia that is a deadly and dreaded form. You can't see the sporocysts in that type in our microscopes because they are super tiny. I think your cham has Eimeria type Coccidia. But in any case, both Eimeria and Isospora are treated the same way. Hope this help....

Thanks Paradlis Girl, that was super informative.:cool: I am turned on by your big brains :p jk..kind of

- Eimeria Coccidia can be treated with Albon?
- What is the best way to clean a cage with a chameleon who has coccidia other than throwing everything away?
- will bleach kill coccidia on equipment?
 
- What is the best way to clean a cage with a chameleon who has coccidia other than throwing everything away?

Other than that, the unconfirmed way is to use steam cleaner set to the highest setting. You want it hot since coccidia will die from boiling water. Do realize it's not yet researched or proven by data of some kind. Just from couple of bearded dragon keepers suggested me to do it.

You can always scrub with 10% bleach, let it dry, rinse, scrub with soapy water, and rinse. Although it will not kill them, at least the soapy water will loosen up the eggs so you can rinse them away


- will bleach kill coccidia on equipment?

No. as far as i know, not even at the strength of 100%. Only boiling water will kill them during their oocyst period
 
Thanks dodolah.

I always try to boil my stuff whenever moving around chages. Ill be sure to pick up a steam cleaner on the way home. thanks for the advice.
 
How effective is it to poor boiling water over everything? It's kinda hard to break aeverything down and get it on a pot on the stove. I'd like to scrub with 50/50 bleach solution and then rinse with boiling water.

Is it worth it? Or by the time the water cools as it hits a surface and spreads out does it lose it's effectiveness?
 
Killing Coccidia.........

I've been keeping reptiles for longer than I care to admit.....I've always used a method of soaking things in a very strong bleach/dish soapy water mix and then blasting/rinsing with a hose jet sprayer. The bleach kills bacteria and other nasty things. It also loosens organic material (including the oocysts) far better than plain soap and water does. The jet water spray from a hose rinses it all away. If a plant can't survive that process it goes into the garbage. I've had great success in eliminating all manner of persistant parasites with this method. I may get a steam cleaner. That would be great for cages and branches. It would be a killer for plants though. Another thing I do since I am home most of the time....I encourage pooping of the affected chameleon outside of the cage. In most cases I keep an acrylic Kritter Keeper with a paper towel bottom and branch handy. Most of the time my chams poop after misting/showering and before eating. So I shower the cham and get it to drink some water in the morning. Then I put it in the Kritter Keeper until it poops. Then I wash off the cham before putting it back in it's cage. Then I disinfect the Kritter Keeper with bleach and have it ready for the next day. This process is a bit of a pain, but it really keeps the cham from reinfecting it's cage during the whole treatment process. You would have to figure out your own cham's pooping "routine" to do this. Another thing....consider turning off the auto mist equipment during treatment. Mist by hand so you are not misting infected poops. Just imagine the spread of oocysts if the cham poops while you are gone and the auto mister mists it and causes dripping of oocysts.....Yuck :(
 
I just turn the water heater to max and throw everything in the tub, and try to soak everything as much as i can. afterwards ill use the steam cleaner to try and work with that.
 
I've been keeping reptiles for longer than I care to admit.....I've always used a method of soaking things in a very strong bleach/dish soapy water mix and then blasting/rinsing with a hose jet sprayer. The bleach kills bacteria and other nasty things. It also loosens organic material (including the oocysts) far better than plain soap and water does. The jet water spray from a hose rinses it all away. If a plant can't survive that process it goes into the garbage. I've had great success in eliminating all manner of persistant parasites with this method. I may get a steam cleaner. That would be great for cages and branches. It would be a killer for plants though. Another thing I do since I am home most of the time....I encourage pooping of the affected chameleon outside of the cage. In most cases I keep an acrylic Kritter Keeper with a paper towel bottom and branch handy. Most of the time my chams poop after misting/showering and before eating. So I shower the cham and get it to drink some water in the morning. Then I put it in the Kritter Keeper until it poops. Then I wash off the cham before putting it back in it's cage. Then I disinfect the Kritter Keeper with bleach and have it ready for the next day. This process is a bit of a pain, but it really keeps the cham from reinfecting it's cage during the whole treatment process. You would have to figure out your own cham's pooping "routine" to do this. Another thing....consider turning off the auto mist equipment during treatment. Mist by hand so you are not misting infected poops. Just imagine the spread of oocysts if the cham poops while you are gone and the auto mister mists it and causes dripping of oocysts.....Yuck :(

That seems like alot of work, but I am willing to go through with it before i end up spreading it to my other herps. Seems like a real quarentined way of elminating the coccidia. thanks again ParadlisGirl for the knowledge and info. :)
 
I have also been using that Purell hand sanitizer stuff. how good is that at elimitaing and transferring bacteria? I'm guessing it would do no good for coccidia??
 
I have also been using that Purell hand sanitizer stuff. how good is that at elimitaing and transferring bacteria? I'm guessing it would do no good for coccidia??

Alcohol can kill lots of stuff. But, as far as i know, it needs to be in contact for at least half an hour. Since alcohol evaporates too soon, it become highly ineffective.

Boiling water does the job but unfortunately you might kill the plants in the process.
So far, I now extensively use a steam cleaner for my chams.

Plus, they are really fun to use. Swoooooshhhhh!!!! Die you parasites!!! Die!!!
:D:D
 
Alcohol can kill lots of stuff. But, as far as i know, it needs to be in contact for at least half an hour. Since alcohol evaporates too soon, it become highly ineffective.

Boiling water does the job but unfortunately you might kill the plants in the process.
So far, I now extensively use a steam cleaner for my chams.

Plus, they are really fun to use. Swoooooshhhhh!!!! Die you parasites!!! Die!!!
:D:D

haha, is there a particular brand you would recommend using for the steam cleaner? i am shopping online right now and am reading alot of bad reviews!
 
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